Kamis, Januari 23, 2020

Vol. 6, No. 2 (2019)

Indo. J. Phar. Scie. Tech. Vol. 6, No. 2, 52-94 (2019).

Comparative Study of Antioxidant Phycocyanin Extracts Activity between S. platensis with S. fusiformis Using DPPH Method

Dwi Margiati*, Danni Ramdhani, Asri P. Wulandari


Phycocyanin is Spirulina’s content which has antioxidant activity to inhibit oxidation process or formation of free radicals that play a role in the initiation high-degenerative diseases. This research is aim to determine the best antioxidant activity of S. platensis with S. fusiformis through the best extraction methods of Spirulina cell between distilled water and PBS (Phosphate Buffer Solution). This research method existed Spirulina cell extraction used distilled water and PBS, and then followed by fractionation and dialysis phycocyanin extract, afterward tested the antioxidant activity used DPPH method. The result of extraction used PBS with a salt concentration of ammonium sulfate 30% produced the best purity value of phycocyanin extract of both types of Spirulina. Results of tested the antioxidant activity, S. fusiformis has the best antioxidant activity compared with S. platensis with a value of 311.43 ppm and 387.36 ppm, respectively.


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Evaluation Patch of Rhizoma Extract Kencur (Kaempferia galanga L.) as Anti-Inflammatory with Enhancer

Hesti Riasari*, Revika Rachmaniar, Sri Wahyuni


Kencur (Kaempferia galanga L.) is a family of Zingiberaceae. Several studies have shown that kencur can help reduce inflammation because kencur is known to contain anti-inflammatory compounds, namely marker compounds from flavonoids, kaempferol. For the development of pharmaceutical preparations, research on anti-inflammatory plasters containing 96% ethanol extract, n-hexane extract, ethyl acetate extract and 70% ethanol extract from ginger rhizome with the addition of penetration enhancer (enhancer), namely propylene glycol. This anti-inflammatory plaster was tested for its activity in 5 groups of Wistar strain rat feet which had been induced 1% carrageenan (negative control); positive control (diclofenac sodium), ethanol96% extract, n-hexane extract, ethyl acetate extract and 70% ethanol extract from kencur rhizome and compared with plaster of kencur rhizome ethanol extract without enhancer. The results showed the effect of adding enhancers 30 minutes after administration. 96% ethanol extract and ethyl acetate extract had reduced inflammation by 79.99% in rat test animals compared to plaster ethanol extract of rhizome kencur without the addition of enhancers.


| Full HTML | PDF | 59-64 |

Molecular Dynamics Simulation of Estrogen Receptor Alpha Against Andrografolid as Anti Breast Cancer

Doni Dermawan*, Riyadi Sumirtanurdin, Deti Dewantisari


Breast cancer is the most common cancer suffered by women with 1.67 million new cases in the world by 2012 with a mortality rate of 12.9%. Tamoxifen is a standard therapy for breast cancer but can cause endometrial and thromboembolic cancer. Andrografolid is an active compound from Andrographis paniculata which has antiproliferation activity of MCF-7 breast cancer cells with IC50 was 61.11 μM. The purpose of this study was to design andrographolide modification structures as human estrogen receptor alpha (hER-α) antagonists. Molecular docking simulation results showed that the andrographolide and AND5 have free binding energy (ΔG) values of -9.65 kcal/mol and -12.43 kcal/mol, respectively, and hydrogen bonds were formed with Gly521, Asp351, and Met343. The ΔG value of ANDS was lower than tamoxifen (-11.40 kcal/ mol). Pharmacophore modeling results showed that andrographolide and AND5 had a high pharmacophore- fit value of 46.39% and 63.47%, respectively. Molecular dynamics simulation showed that the hERα-AND5 system has a value of ΔGTOTAL = -50.52 kcal/mol compared to the hERα-estradiol system with a value of ΔGTOTAL = -40.86 kcal/mol. These results suggested that AND5 has better affinity for hERα compared to estradiol so that AND5 is a very promising anti breast cancer agent.


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Optimization of Sample Preparation Methods on Formaldehyde Analysis in Meatball with Schryver’s Method

Febrina A. Saputri*, Nur S.B. Rosli, Wiwiek Indriyati


Meatball is one of Indonesian’s favorite food. However, the fraudulent traders often add carcinogen compounds, formaldehyde to ensure the meatball is not easily rotten. The purpose of this study was to find out the best sample preparation method for formaldehyde analysis in the meatball. The preparation methods were the distillation, hot immersion, and cold immersion. Formaldehyde was then analyzed by Schryver’s method. UV-Vis spectrophotometer was used for quantitative analysis. The distillation method yielded the highest recovery (100.64%) compared to hot immersion (98.11%) and cold immersion (94.17%). The best method was used to test the meatball samples that have been collected around Jatinangor, Indonesia. The results of the qualitative analysis for five samples of meatball showed that two samples containing formaldehyde with the highest concentration of formaldehyde in the sample B2 (14.72 ppm) and followed by B5 (8.77 ppm).


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Antihyperlipidemia and Antihyperglycemic Studies of Arcangelisia flava (L.) Merr. Phenolic Compound: Incorporation of In Vivo and In Silico Study at Molecular Level

Wahyuning Setyani*, Hanny Setyowati, Dwi H. S. Palupi, Hanievia Rahayunnissa, Maywan Hariono


Yellow root had been extensively studied its potential as herbal medicines in many pharmacologic activities due to its alkaloid content namely berberine. To date, there is no study of the yellow root as source of flavonoid to have anti-hyperglycemia and anti-hyperlipidemia effect. This present study focuses on flavonoid fraction being investigated its in vivo potential to reduce blood glucose level (165 mg/dL at 28 mg/kg of a fraction), peroxidase inhibition, and catalase activation. The extract was obtained from the fresh yellow root, using the re-maceration method. The evaluation of anti-hyperglycemic and anti-hyperlipidemia activity was done using the fraction form of yellow root. The isolation of phenolic compounds was done by radial chromatography (chromatotron). The isolated compound was then structurally and computationally characterized using NMR and molecular docking. The fraction reduced the activity of peroxidase (6.33%) at 14 mg/kg and increase catalase activity (15.74%) at 28 mg/kg of body weight of fraction, respectively. An effort to isolate the flavonoid in the fraction reveals a rutin-like structure, a flavonoid glycoside. This result guides the utilization of rutin and its aglycon as the model of lipid peroxidase inhibitor and catalase activator upon in silico molecular docking.

| Full HTML | PDF | 84-94 |