Rabu, September 26, 2018

Vol. 5, No. 1 (2018)

Indo. J. Phar. Scie. Tech. Vol. 5, No. 1 (2018).
https://doi.org/10.24198/ijpst.v5i1


Effect of Carrageenan as Gelling Agent on Tocopherol Afcetate Emulgels

Meiti Rosmiati, Marline Abdassah, Anis Yohana Chaerunisaa

Abstrak

Vitamin E (Tocopherol acetate) is used in both oral and topical dosage. The aim of the research was to study different concentration of Carrageenan, as gelling agent in Tocopherol acetate Emulgels. This experimental study was initiated by emulgel formulation using different concentration of carrageenan as gelling agent, as much as 0,5% (F1), 0,75% (F2), 0,85% (F3), 0,95 % (F4), 1% (F5), 1,125% (F6), 1,25% (F7), 1,5% (F8) and 2% (F9). Tween 20 and Span 20 were used as emulsifiers (1 and 1,5 %), Liquid paraffin as oily phase (7,5%), Propylen glycol as humectant (10%), propyl and methyl paraben as antimicrobial preservative (0,01% and 0,03%). The physical investigation of emulgel were included pH, spreading test, viscosity and freeze thawtest. The results for promising formula F5 showed that physical parameters of emulgels were in the range of requirement for topical dosage form.

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Stability of Eleutherine americana (L.) Merr. Extract as Lipstick Colorants as the Change of Temperature, Time, Storage Condition and the Presence of Oxidator

Dewi Mayasari, Taofik Rusdiana, Yulien Ratu Kania, Marline Abdasah

Abstrak

Umbi bawang tiwai (Eleutherine americana (L.) Merr ) merupakan tanaman khas masyarakat Kutai yang secara empiris banyak digunakan sebagai obat anti-bakteri dan perwarna makanan. Pada peneli- tian ini dikaji stabilitas ekstrak Umbi bawang tiwai Eleutherine americana (L.) Merr. untuk mengetahui kestabilan dari ekstrak tersebut. Uji stabilitas tersebut dilakukan dalam dua pelarut yaitu akuades dan etanol meliputi pengaruh suhu (25°C, 50°C, 80°C), pH (3, 5, 7), oksidator selama 6 jam, kondisi penyimpanan suhu ruang dan suhu kulkas selama 24 jam dan waktu penyimpanan selama 2 minggu. Absorbansi ekstrak dalam pelarut etanol dan akuades mengalami penurunan pada perubahan suhu, waktu penyimpanan, adanya oksidator dan pada perubahan pH absorbansi meningkat, sedangkan pada kondisi penyimpanan, kondisi gelap pada suhu ruangan memiliki absorbansi tertinggi dibandingkan dengan kondisi penyimpanan lain.

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Simultaneous Estimation of Ketorolac Tromethamine and Tiemonium Methylsulphate in Biofluids by a Validated UPLC Method

Mohammad Nasir Uddin*, Monir Uddin, Md. Touhidul Kabir

Abstrak

An Ultra Performance Liquid Chromatography (UPLC) method has been developed for the simultaneous determination of ketorolac tromethamine (KTR) and tiemonium methylsulphate (TMS) in pharmaceutical and bio-samples. Calibration curves were linear with correlation coefficient 0.9997 at a studied concentration range of (0.1-10 μg mL-1) for both drugs. The method was reproducible with relative standard deviation (RSD) for intraday precision was 0.32-0.99% for KTR and 0.40-1.01% for TMS and inter day precision was 0.51-2.77% for KTR and 0.41-2.26% for TMS. The mean recovery of the intraday assay was 99.18-103.34% for KTR and 99.14-101.76% for TMS and inter-day assay at six different days was 99.39-102.04% for KTR and 98.08-103.78% for TMS. The limit of detec- tion (LOD) and limit of quantitation (LOQ) were 0.125 and 0.41 μg mL-1, respectively for KTR and 0.150 and 0.50 μg mL-1, respectively for TMS. The method was successfully validated as per the ICH guidelines can be conveniently employed for routine quality control analysis of KTR and TMS in pharmaceutical formulations and biological samples without any interference.

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Formulation of The Body Scrub Cream from Purple Sweet Potato (Ipomoea batatas L.)

Made Agus Gelgel Wirasuta*, Ni Komang Dewi Triastuti, Kadek Sintia Deviyanthi, Dyah Aryani Sartika, Putu Dyah Utari

Abstrak

Anthocyanin is an active compound of purple sweet potato, has effect as anti-oxidative, anti-inflam- matory, and anti-itching. It make them interesting ingredients for skin treatment. The bio-antioxidant body scrub is one of the popular cosmetic products in SPA industry. Their stability property is one crucial factor to develop a purple sweet potato body scrub cream. The steam and oven heating of tuber were involved on the scrub powder preparation. The oven and non-heating preparation scrub powder provided fine-sized powder more than 50%, but the steaming produced granular particle distribution. Total anthocyanin content in scrub grains was 326.8 mg/100 g, 103.3 mg/ 100 g and 34.4 mg/100g for steam preparation, oven and non-heating, respectively. The steamed scrub powder preparation method produced stable anthocyanin content in body scrub cream for 25 days observation.

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TLC Profile and Activity Test of Secondary Metabolites Aspergillus flavus “In-Habiting” Queen Termite’s Nest Macrotermes gilvus on Enriched Media

Yohannes Alen*, Rezki Amelia dan Akmal Djamaan

Abstrak

Peneliti terdahulu telah berhasil menapis empat jamur yang hidup disarang ratu termite Macrotermes gilvus Hagen., salah satunya Aspergillus flavus. Jamur ini bertahan menghasilkan senyawa metabolit golongan fenol pada media SDA hanya pada ekstrak subkultur biakan pertama dan kedua, berangsur hilang pada biakan berikutnya, hal ini diduga terjadi karena perbedaan habitat tumbuh. Oleh karena itu dilakukan pengayaan media dengan bahan sarang ratu termite yang bertujuan untuk mengetahui apakah senyawa metabolit golongan fenol dapat kembali terbentuk atau justru membentuk senyawa metabolit baru. Pengayaan dilakukan menggunakaan empat konsentrasi media berbeda (0,25; 0,50; 0,75 dan 1 g/mL). Hasil yang diperoleh menunjukkan pengayaan media SDA dengan sarang 1 g/mL memberikan pertumbuhan jamur paling optimal. Jamur subkultur biakan ketiga dibiakkan pada media dan menjadi biakan keempat. jamur dibiakkan hingga subkultur kesepuluh, dilakukan ekstraksi dan fraksinasi terhadap masing-masing biakan. Berdasarkan analisis profil KLT fraksi etil asetat jamur dengan fase diam silika gel GF254nm dan fase gerak kloroform : metanol (9:1), senyawa metabolit golongan fenol belum terbentuk hingga subkultur kesepuluh, namun terbentuk 6 noda baru golongan terpenoid. Hasil kromatografi kolom didapatkan 10 sub-fraksi. Uji aktivitas dilakukan dengan metode difusi terhadap 12 bakteri uji dan 3 jamur uji. Noda 2, 5, dan 6 (Rf 0,84; 0,36; 0,26) diduga meng- hambat pertumbuhan Pseudomonas aeruginosa ATCC 27853, noda 3, dan 4 (Rf 0,74; 0,52) diduga menghambat pertumbuhan Micrococcus luteus ATCC 10240. Penggunaan media SDA yang diperkaya sarang ratu termite mempengaruhi pembentukan metabolit sekunder jamur Aspergillus flavus.

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