Kamis, Juli 19, 2018

Vol. 5, No. 2 (2018)

Indo. J. Phar. Scie. Tech. Vol. 5, No. 2, 43-75 (2018).

Antioxidant Activity of Combination Ethanol Extract of Turmeric Rhizome (Curcuma Domestica Val) and Ethanol Extract of Trengguli Bark (Cassia Fistula L) with DPPH Method
Triyono Triyono, Anis Yohana Chaerunisaa, Anas Subarnas

Turmeric rhizome (Curcuma domestica Val) and Trengguli bark (Cassia fistula L) contain antioxidant compounds which can be determined by 1,1-Diphenyl-2- Pycrylhydrazyl (DPPH) free radical inhibition method. This research was conducted to determine DPPH free radical inhibition by ethanol extract of turmeric rhizome, the ethanol extract of trengguli bark, and a combination of turmeric rhizome extract – trengguli bark extract (1:1,5) with ascorbic acid as a comparison. Identification of secondary metabolite classes is performed by phytochemical screening. Antioxidant activity was performed by inhibition of free radical color of DPPH using UV-Vis spectrophotometry. The study showed IC50 value of ascorbic acid, as a comparasion, is 3,14 μg/mL. While ethanol extract of trengguli bark has the best antioxidant activity with IC50 value 10,98 μg/mL compare to combination ethanol extract of turmeric rhizome – trengguli bark (1 :1,5) and ethanol extract of turmeric rhizome with IC50 value is 13,70 μg/mL and 41,95 μg/mL, respectively.


| Full HTML | PDF | 43-48 |

Formation of Andrographlolide-BetaCyclodextrin Inclusion to Increase Solubility and Dissolution Rate
Bayu Febram Prasetyo, Ietje Wientarsih, Dondhin Sajuthi, Vetnizah Juniantito

Andrographolide (AG) is a pure isolate of the chemical synthesis of sambiloto (Andrographis paniculata Nees.) which has various pharmacological properties such as anti-inflammatory and antimicrobial. In order to improve the ability of AG to penetrate the membrane in transdermal use, an inclusion complex was formed using beta-cyclodextrin (BCD) by modifying the physical chemistry properties of AG, particularly the solubility in its base, partition, and distribution on the skin, as well as by changing the permeability of the stratum corneum. The inclusion complexes of AG with BCD were prepared by the solvent evaporation method in the mole ratios of 1:1, 1:2, and 2:1. The solid of the yield of AG inclusion complex in BCD has been tested with a solubility test until it reached equilibrium at 37 ± 5°C for 24 hours and In vitro dissolution rate test using the II USP method (paddle type), then compared to a single AG compound, and a physical mixture of AG-BCD. Based on the solubility and dissolution rate tests, it showed that the formation of AG-BCD inclusion complex was obtained at the mole ratio of 1:2. Moreover, the AG solubility increased 38 timesand the dissolution rate in the 60th minute increased twice  in the inclusion complex with BCD.


| Full HTML | PDF | 49 – 54|

Isolation and Phisicochemical Characterization of Microcristalline Cellulose from Ramie (Boehmeria nivea L. Gaud) Based on Pharmaceutical Grade Quality
Desy Nawangsari, Anis Yohana Chaerunisaa, Marline Abdassah, Sriwidodo Sriwidodo, Taofik Rusdiana, Linda Apriyanti

Microcrystalline cellulose is the most used material for medicine, which able to be found in fibrous plants. Microcrystal celluloses are being used as filler or binder in dosage formulas in tablets and capsules. This research aimed to produce microcrystalline cellulose from ramie based on pharmaceutical grade parameters. Research method include hemp fiber preparation, α-cellulose isolation, microcrystalline cellulose production, and microcrystalline cellulose characterization which compare with Avicel® PH 102 . Result shown microcrystalline cellulose yield is 57.26%. The result of physicochemical characterization that can comply the specifications of pharmaceutical grade as a pharmaceutical excipient.


| Full HTML | PDF | 55-61 |

Isolation of Secondary Metabolite A. niger “In-Habiting” Queen M. gilvus Hagen.’s Nest
Yohannes Alen, Atika Melati, Gemmy Sarina, Akmal Djamaan

Aspergillus niger is pathogen fungi that can live in various locations and can live contiguous with many hosts, one of them is queen termite’s nest. The aims of the study were to isolated the secondary metabilite of A.niger. Extraction proccess of secondary metabolite compounds was carried out by maceration method using methanol solvent. Based on that proccess, methanol extract was be yield 4,32% sample weight. Fractination proccess was carried out in the separating funnel using ethyl acetate solvent, which ethyl acetate fraction was be yield 14.39% methanol extract. Separation of the compounds was carried out by column chromatography method using n-hexane and ethyl asetate eluents. Purification of the compounds were done by recrystallization method using n-hexane and ethyl asetate. Two secondary metabolite compounds were successfully isolated from ethyl acetate fraction of the methanolic extract of fungus A. niger “In-Habiting” queen termite’s nest M. gilvus Hagen. Based on organoleptic examination, the compound signed AM-12-22-01 is 35 mg, white needle crystals, melting point 151-153 oC. While, the AM-12-60-01* is 15 mg, white needle crystals, melting point 91-93 oC. Based on the chemical analysis, thin layer chromatography, ultraviolet and infrared spectra data it was identified that AM-12-22-01 and AM-12-60-01 were a phenolic compounds.


| Full HTML | PDF | 61-70 |

Effect of Fermentation Time and Cassava Varieties on Water Content and the Yield of Starch from Modified Cassava Flour (MOCAF)
Nurud Diniyah, Achmad Subagio, Riri Nur Lutfian Sari, Pradiska Gita Vindy, Alif Ainur Rofiah

MOCAF (modified cassava flour) is a high potential food product. This product can be used to replace wheat flour in the manufacture of various food products. However, there is no characterization of starch properties of MOCAF. The efforts to improve the quality of starch from cassava starch by fermentation process using a combination of enzymes and microbes  from Lactic acid bacteria.The purpose of this research is to determine the influence of fermentation time to yield and moisture content. Single factor randomized block design with 3 (three) levels i.e fermentation time (0, 12, and 24 hours) with two group namely varieties of cassava Cimanggu and Kaspro. Cimanggu and Kaspro cassava varieties were used to compare the properties of starch due to different fermentation time (0, 12 and 24 hours) with triplicate. The results showed the yield and moisture content were affected by fermentation time. Average moisture content ranged from 13.3 to 13.8% with the starch yield is 38-59%. Application of starch MOCAF can be used for food, cosmetics, and medicines industry.

| Full HTML | PDF | 71-75 |