Determination of Salbutamol and Guaifenesin in Mixture Using Zero- Crossing Wavelength Measurement

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Indo. J. Phar. Scie. Tech. Vol. 2, No. 2, 45-48 (2015). http://dx.doi.org/10.15416/ijpst.v2i1.7809
Entris Sutisna1, Farida Fauzia1, Ida Musfiroh2, Shelvy E. Suherman2

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1Sekolah Tinggi Farmasi Bandung, Bandung, West Java, Indonesia
2Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, West Java, Indonesia
Korespondensi: idamusfiroh@yahoo.com (ida musfiroh)

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Abstrak/Abstract

Kata Kunci: Guaifenesin, salbutamol, spektrofotometri derivatif, zero-crossing

 

Introduction

Derivative spectrophotometry is a technique based on derivative spectra of a basic, zero-order spectrum.1 The results of derivatization of function described a run of absorbance curve is called the derivative spectrum. It has found a wide application in the quantitative chemical analysis.2 It can be used for multi component analysis, such as determination of drugs mixtures.3

An example of drugs in mixture are salbutamol and guaifenesin in syrup. Salbutamol in sodium hydroxide (NaOH) shows maxima absorption (λmax) at 295 nm, 4 while guaifenesin is at 273 nm.This paper described application of zero-crossing wavelength (λzero-crossing) measurement to determine salbutamol and guaifenesin in syrup.

Methods

Instrument that used was ultraviolet spectrophotometer (Shimadzu 1700) and chemical glasswares. Materials that used was salbutamol and guaifenesin (BFPI), methanol, HCl 0.1 N, NaOH 0.1 N.

This research was conducted with raw materials analysis. Raw materials of salbutamol and guaifenesin were analyzed according to Indonesian Pharmacope 5th edition that included solubility test and identification reaction.6

Salbutamol and guaifenesin in syrup were analyzed. The steps were preparation  of standard solutions, determination of λma and λ zero-crossing, preparation of standard curve at each λ zero-crossing, made a series of concentration from standard solution, the validation of analytical method, and then salbutamol and guaifenesin in syrup were determinated.

Salbutamol standard solutions 1000 ppm was made by weighing accurately 0.01 g of salbutamol and dissolving it in ethanol 10 mL. A diluted solution of 100 ppm was prepared by pipetting 1 mL of the solution and diluting it with NaOH 0.1 N into 10 mL. Guaifenesin standard solution 1000 ppm was made by weighing accurately 0.01 g of guaifenesin and dissolved it in ethanol 10 mL.

Determination of λ max and λzero-crossing of salbutamol and guaifenesin were obtained by deriving the absorbance of the normal spectra towards the wavelength (dA/dλ).7

Salbutamol and guaifenesin standard curves were obtained by measure (dA/dλ) of the series of standard solutions at λ crossing of the other compounds.7 Linear regression equations were calculated.

The concentration series of salbutamol standard solution were 0.2; 0.4; 0.6; 0.8; 1.0; 1.2; and 1.4 ppm. It was made from salbutamol standard solution 1000 ppm. The series concentrations of guaifenesin standard solution were 9, 18, 27, 36, 45, 54, and 63 ppm. The series concentrations were made from standard solution of guaifenesin 1000 ppm.

Table 1 Identification of Salbutamol and Guaifenesin6

Figure 1 UV Spectrum of Salbutamol (a) and Guaifenesin (b), UV First Derivative Spectrum of Salbutamol (Blue) and Guaifenesin (Yellow) (c)

salbutamol and guaifenesin (80%, 100%, 120%)9 then dissolved into sugar solution. All concentrations of each compound were calculated using linear regression equation to obtain accuracy and precision. LOD and LOQ were calculated by measuring the absorbance of blank solution.8

Salbutamol and guaifenesin in syrup were determined by weighing accurately mixture of salbutamol (4 ppm), guaifenesin (18 ppm), and syrup then measured its absorbance at λzero-crossing.

Results

Salbutamol and guaifenesin were analyzed by according to Indonesian Pharmacope 5th edition. Table 1 shows the result of salbutamol identification.6

Ultraviolet spectrum of salbutamol and guaifenesin are showed in Figure 1a and 1b. The first derivative spectrum of salbutamol and guaiafenesin showed in Figure 1c. Salbutamol and guaifenesin standard curve were obtained by measuring (dA/dλ) of the series of standard solutions at λ zero-crossing of the other compounds. The calibration curve of salbutamol and guaifenesin showed in Table 3.

Table 3 Calibration Curve of Salbutamol and Guaienesin

Discussions

Salbutamol shows maxima at 295 nm in NaOH4 (A= 0.420), while guaifenesin is at 273 nm5 (A= 0.458) (Figure 1a and 1b). Zero-crossing wavelength of salbutamol is 246.2 nm and guaifenesin is 270.2 nm (Figure 1c).

Linearity of the calibration curves (Table 3 and 4) is presented by the linear regression equation and coefficient of correlation (r).8 Linear regression of salbutamol is y=0.219x+0.006 (r=0.993), while for salbutamol is y=0.012x+0.022 (r=0.997). Results of salbutamol and guaifenesin analysis are written in Table 5. Accuracy of the method was determined by calculating % recovery8 and the result was 95.96% (salbutamol) and 93.94% (guaifenesin).

The method precision was determined by calculating coefficient of variance8 and the result was 0.995% for salbutamol and 0.2087% for guaifenesin.

Conclusions

Zero-crossing derivative method could be applied to determine salbutamol and guaifenesin in mixture. Limit of detection of salbutamol and guaifenesin are 0.05528 ppm and 9.443 ppm, respectively, while limit of quantification are 0.18427 ppm and 31.477 ppm.

Refferences

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